The Journal of Industry and Technology

Industrial Analytical Instrumentation

Euro Technology

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Life sciences and clinical instrumentation

Dolomite's microfluidics technology
helping to advance antibody therapeutics

Dolomite's droplet generation system is helping AbVitro to identify potential therapeutic targets using its AbPairâ„¢ technology. Dr Adrian Briggs, Director of Molecular Biology R&D at AbVitro, commented: "Our aim is to develop new potential targets and therapies for a wide range of diseases, taking advantage of the specificity and sensitivity of the human immune system. To identify complete functional antibodies - pairing the correct heavy and light chains - we use a molecular barcoding technique that requires isolation of individual B or T cells. Achieving this with a flow sorter and microplate format is extremely slow and laborious, and so we looked for a reliable microfluidics solution that would enable much higher throughput."

"We investigated the various systems available, and Dolomite seemed to be the most highly engineered, optimized and mature technology. Very precise control of pressure is crucial to reproducible, reliable droplet formation, and Dolomite offers high quality pumps, flow sensors and microfluidics chips, all fully automated via the Company's software."

"Since its introduction, the system has been in use almost constantly, and we have been very happy with the performance. The system's quartz chip, in particular, is very robust and long-lasting - we have been using the same chips since we first started - and the computer-controlled Mitos P-Pumps with flow sensors offer outstanding reproducibility. Using this set-up, we can process in excess of a million cells per experiment, allowing us to obtain over 300,000 full-length, natively-paired antibodies from a single sample. This kind of throughput would be impossible with traditional methods, as the cost and time required would be prohibitive, as well as having a poor success rate. Having a robust emulsion hardware solution has saved us significant research and development time, allowing us to proceed to product development and partnerships in studies of human disease sooner."

The Dolomite Centre Limited in profile
Established in 2005 as the world's first Microfluidic Application Centre, Dolomite focused on working with customers to turn their concepts for microfluidic applications into reality. Today, Dolomite is the world leader in solving microfluidic problems. With offices in the UK and US and distributors throughout the rest of the world, its clients range from universities developing leading-edge analytical equipment, to manufacturers of chemical, life sciences and clinical diagnostics systems.

Dolomite is pioneering the use of microfluidic devices for small-scale fluid control and analysis, enabling manufacturers to develop more compact, cost-effective and powerful instruments. By combining specialist glass, quartz and ceramic technologies with knowledge of high performance microfluidics, Dolomite is able to provide solutions for a broad range of application areas including environmental monitoring, clinical diagnostics, food and beverage, nuclear, agriculture, petrochemical, cosmetics, pharmaceuticals and chemicals. Furthermore Dolomite's in-house micro-fabrication facilities that include clean rooms and precision glass processing facilities allow to prototype and test all solutions rapidly which ensures a faster development cycle and reduces the time to market.

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