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Industrial Analytical Instrumentation

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Microfluidics

Dolomite releases novel droplet-on-demand
sequencing and droplet generation microfluidic system

Microfluidic products expert Dolomite has launched Mitos Dropix, an ingenious droplet-on-demand system capable of easily generating extremely miniaturised droplet compartments with exceptional control over volume, environment and isolation of contents. Meeting the increasing demand for screening massive numbers of biological reactions, increased speed of screening and reduced reagent consumption, Mitos Dropix technology now introduces liquid sampling and processing over a very wide 10 nL - 50 μL volume range utilizing droplet technology.

Mitos Dropix will be invaluable to application areas such as droplet library creation, cell encapsulation, diagnosis screening, synthetic chemistry and drug bioassays, as well as being applicable to high-throughput screening formats.

Mitos Dropix is a compact, off-chip droplet generator that features a patented droplet "picking" technology. It was developed by Dolomite under exclusive sub-licence with Drop-Tech Ltd. having won Dolomite's 2012 Productizing Science® competition. Drop-Tech was formed from an academic collaboration between Cambridge University and Imperial College London and is the exclusive licensee of their patented droplet generation technology used in Mitos Dropix (Patent Pending: PCT/GB2013/051668).

There are 24 wells in total, allowing users to combine multiple reagents to create complex assays, resulting in up to 300 combinations. 8 sample strips can also be inserted into the Dropix Sample Strip Holder thereby creating a 192 well plate configuration which facilitates preparation and allows integration with existing standard fluid handling systems. Mitos Dropix is capable of consistently generating and storing up to 1000 droplets, with accurate user-defined control of droplet size (ranging in volume from a few 10's of nanoliters to a microliter) and frequency (up to 5Hz of one sample) achieved via Dropix PC software.

The following application note demonstrates the capabilities of the Mitos Dropix system for droplet-on-demand sequencing and droplet library generation: View website: http://www.dolomitemicrofluidics.com/images/stories/PDFs/dropix_application_note.pdf

The Dolomite Centre Limited in profile
Established in 2005 as the world's first Microfluidic Application Centre, Dolomite focused on working with customers to turn their concepts for microfluidic applications into reality. Today, Dolomite is the world leader in solving microfluidic problems. With offices in the UK and US and distributors throughout the rest of the world, its clients range from universities developing leading-edge analytical equipment, to manufacturers of chemical, life sciences and clinical diagnostics systems.

Dolomite is pioneering the use of microfluidic devices for small-scale fluid control and analysis, enabling manufacturers to develop more compact, cost-effective and powerful instruments. By combining specialist glass, quartz and ceramic technologies with knowledge of high performance microfluidics, Dolomite is able to provide solutions for a broad range of application areas including environmental monitoring, clinical diagnostics, food and beverage, nuclear, agriculture, petrochemical, cosmetics, pharmaceuticals and chemicals. Furthermore Dolomite's in-house micro-fabrication facilities that include clean rooms and precision glass processing facilities allow to prototype and test all solutions rapidly which ensures a faster development cycle and reduces the time to market.

For further information on Dolomite's range of Multiflux® Connectors and Interfaces as well as a complete portfolio of microfluidic products including chips, pumps, valves and custom devices view website: www.dolomite-microfluidics.com

Dolomite Microfluidics and Sphere Fluidics partner
to deliver world leading droplet based technology

Sphere Fluidics and The Dolomite Centre Ltd. have now signed an exclusive distributor agreement for Sphere Fluidics' trademarked range of unique biochips, surfactants and other specialist chemicals for picodroplet applications. These will be promoted and sold via Dolomite's worldwide sales channels.

Dr Robert Marchmont, Commercial Director of Sphere Fluidics, stated: "We are very impressed with Dolomite's technical, manufacturing and commercial expertise in the area of microfluidics and are now pleased to strengthen our current relationship with this new agreement. Specifically, we see great synergy between our scientific development and their Productizing Science® skills and sales & marketing reach. We are looking forward to further growing our successful partnership." Mr Lee Jeffries, COO of Dolomite, said: "Picodroplets are a fascinating and fast growing area of research, especially useful where tests need to be conducted on picolitres of sample containing, for example, single cells or biologically-important molecules. Sphere Fluidics' scientific know-how and capability in the core areas of picodroplet based technology will enable Dolomite to enhance our market leading microfluidic system solutions, by offering unique capabilities in picodroplet generation, isolation and analysis."

Sphere Fluidics Limited in profile
Sphere Fluidics Limited is an established, Life Sciences company which has developed unique products for use in single cell analysis and characterisation and provides collaborative R&D services in this area.

Sphere Fluidics' technology have patented a novel technology platform that enable ultra-high throughput analysis of single cells and generation of their genetic, transcriptomic and proteomic profiles in miniaturised (pL to nL) volumes called picodroplets.

Uniquely, these systems enable the study of different, large biomolecules that interact with or are secreted by single cells. The latter application is very powerful as many human biotherapeutics, industrial enzymes (isolated mainly from micro-organisms) and around 15% of the Human Proteome are secreted, so cannot readily be analysed using conventional technology. Exosomes, disease-related proteins, novel biomarkers and potential diagnostics, are also released from cells and should also be measurable in picodroplets.

Sphere Fluidics' technology enables: study of the mechanism of cancer cell resistance to chemotherapy, generation of new enzymes and identification of novel microbial strains. Sphere Fluidics has established multiple commercial partnerships, received business angel investment and together its' Scientific Founders and the Company have received almost £10 million in science and business funding to date.

The Company has the following USPs
§ One of the very few teams in the world with expertise in single cell analysis and picodroplet technology.
§ Leading-edge, patented technology and proprietary know-how enables analysis and detection of single cells and their biomolecules.
§ Extensive management experience in successfully commercialising innovative science and engineering.
§ Expertise in new science with key applications for novel biopharmaceutical and biosimilar discovery.
§ Our novel process for single cell analysis and characterisation provides significant saving of time, resource and money compared to conventional workflows

For further information, view website: www.spherefluidics.eu 

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